Isolation and identification of nematodeantagonistic. Pdf biodegradation activity of aspergillus niger lipase. Aspergillus and aspergillosis resource provides secondary metabolite information from aspergillus species, but redundancy exists and sources remain undefined with major focus on aspergillosis 50 and is currently merged with central aspergillus data repository cadre 51. This study was carried out in isolation and screening of xylanase enzyme from the aspergillus niger fungus.
Pdf isolation of aspergillus niger strains from soil and. Aspergillus niger is a fungus and one of the most common species of the genus aspergillus it causes a disease called black mold on certain fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. Isolation and identification of microorganism from polyhouse. The study collected soil samples from three 3 different mechanic workshops soil. Aspergillus niger was among the earliest microorganisms used for the conversion of prochiral sulfides to chiral sulfoxides, and a model fig. In table2 shows that aspergillus niger and penicillium sp had the highest. Tlc showing the metabolites formed during the degradation of endosulfan by aspergillus. The present study deals with isolation of amylase producing bacteria from soil. One hundred and ninetyseven cultures were obtained from different soil samples by serial dilution method using malt extract agar medium. Aspergillus niger is also cultured for the extraction of the enzyme, glucose oxidase, used in the design of glucose biosensors, due to its high affinity for. One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium. Response of aspergillus niger strain, varied with their source of isolation.
Samples were obtained from the soil surface and sowed in sabouraudglucose agar, adding streptomycin and gpenicillin. This appears to be the first report of the isolation of methylated citric acid derivatives from a strain of filamentous fungus. Aspergillus niger growing from goldmining solution contained cyanometal complexes, such as gold, silver, copper, iron, and zinc. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Attachment i final risk assessment for aspergillus niger february 1997 i. The maximum concentration of xylanase enzyme found at. Isolation and identification of keratinophilic fungi from. Isolation, identification, and characterization of an. Here, we report the production of dimethyl citrate 1, trimethyl citrate 2 and dimethyl oxalate 3 by a strain of fungus identified as aspergillus niger van tiegh. After 72 h of culture, at least one strain of aspergillus was isolated from 140 samples 80. Hawranik and sorensen characterized 1,3dimethyl citrate from a. About 104 different strains of aspergillus niger were isolated from soil collected from different areas of lahore by serial dilution method on xylene agar malt extract medium.
Production and optimization of phytase by aspergillus niger. Aspergillus fungi definition, characteristics, types and. Log 10 of total count cfug of aspergillus species in soil samples collected from poultry farms. In this study the isolated fungi were identified on the basis of cultural, microscopic and morphological characteristics fig. Isolation of thermophilic aspergillus one gram soil sample was taken and suspended into 10 ml sterile distilled water. The strains of aspergillus niger can be isolated from soil, water and air wu et al. Various physical and chemical conditions were employed in examining their influence on cellulase production by the selected fungal strain. From the fungal isolates the most of the species belonging to the genera aspergillus and mucor were dominant. Aspergillus niger, aspergillus nidulans, aspergillus terreus, penicillium chrysogenum, penicillium frequentans. Isolation and identification of soil fungi from kadegaon. Isolation and characterization of citric acid producing. Isolation and identification of aspergillus section. Throughout this study, the fungal strain aspergillus niger was maintained under the submerged condition for a period of 7 days at 120 rpm rotational speed. Biodegradation of organochlorine pesticide, endosulfan, by a.
However, different techniques of production are continuously being studied showing new perspectives for the production of citric acid. Paddy, corn, ragi, red gram, cotton and sugarcane were observed. The isolated fungi such as aspergillus sparsusi, a. Soil samples from compost and heaps 1 g were suspended in 10 ml of. Pdf isolation of fungi seasonally from soil, air and water.
Identification key for aspergillus species isolated from. Fiftynine lipaseproducing fungal strains were isolated from brazilian savanna soil by employing enrichment culture tecniques. Keratinolytic activity of five aspergillus species. The aliquots were cultured for fungus on czapek dox agar nano 3 2. In addition to producing extracellular enzymes and citric acid, a. Isolation and screening of alkaline lipaseproducing fungi. Isolation and identification of microorganism from.
Isolation and identification of soil mycoflora in different crop fields at salur mandal. Isolation, characterization and identification of microorganisms from spoilt carrots obtained from ose market onitsha, nigeria article pdf available january 2016 with 3,443 reads how we. Aspergillus species from the soil in a 7 ml sterile polystyrene tube, 1 g of soil was suspended in 3 ml sterile of 0. The isolation of citric acid derivatives from aspergillus. This was performed by the serial dilution spread plate technique. Extraction of fermentation cultures revealed the production of significant levels of dimethyl citrate 1 and trimethyl citrate 2, as well as a small amount of dimethyl oxalate 3. Keratinolytic activity of five aspergillus species isolated. Aspergillus, which is a ubiquitous fungus, rarely causes esophagitis.
Isolation and characterization of aspergillus niger nbc001. Its 28s rdna sequence also had some significant identity 90% with. Aspergillus niger isolated from contaminated soil following a. Pdf isolation, characterization and identification of. Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. The composition of the media used in the cultivation of the fungi is given in appendix 1.
Isolation and identification of some fungi from certain. Isolation, production and characterization of amylase enzyme. Optimization and production of itaconic acid using. Aspergillus niger, aspergillus candidus, aspergillus flavus, aspergillus wentii, aspergillus cremeus, aspergillus. Like others, aspergillus niger are filamentous fungi, which means that they tend to form filaments hyphae and thus resemble the structure of a plant. This fungus is isolated from agricultural soil, decayed bread and waste material. Jun 30, 2010 aspergillus niger vb07 was isolated from the soil of citrus fruit market used to produce extracellular naringinase in a liquid medium.
Isolation and identification of aspergillus section fumigati. Isolation and characterization of citric acid producing aspergillus niger from spoiled coconut v. Isolation of fungi put 10 gm of the soil samples were diluted in 90 ml of sterile distilled water. Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. In the presnt study, isolation and identification of fungi from marine soil. Aspergillus niger an overview sciencedirect topics. Optimization of cellulase production by aspergillus niger. Isolation, identification, and characterization of an aspergillus niger bioflocculantproducing strain using potato starch wastewater as nutrilite and its application. Isolation and characterization of extracellular protease producing pseudomonas aeruginosa and aspergillus niger from poultry soil uma maheswari. Isolation and identification of antibiotic producing microorganisms.
The isolated keratinophilic fungi were classified into 10 species belonging to 7 genera. Present study describes the isolation, screening and strain improvement of aspergillus niger for enhanced citric acid production in shake flask using ferrocyanide treated cane molasses as carbon source. Joshi2 1department of biotechnology and allied sciences, jayoti vidyapeeth womens university, jaipur 303007, rajasthan, india. Isolation, production and characterization of amylase. Isolation, identification and screening of pectinolytic fungi from different soil samples of chittoor district p lakshminarasimha reddy1 and a sreeramulu1 research paper. Aspergillus niger, tricalcium phosphate, yield, upt ake, ph author for correspondence internat. In this context, solidstate fermentation appears where agroindustrial residues can be supports to the filamentous fungi. Serial dilution agar plating apinis, 1963, warcups soil plate and waksman direct inoculation methods were employed for the isolation of soil microbes. The most common organic acids isolated from aspergillus spp. Aspergillus niger is a haploid filamentous fungus which is used for waste management and biotransformations in addition to its industrial uses, such as production of citric acid and extracellular enzymes. Pdf isolation, optimization and production of cellulase. It is most commonly found in decaying vegetation, soil, or plants, but it cannot be considered particularly dangerous in comparison to aspergillus fumigatus, which is the most prevalent. Isolation, production and characterization of amylase enzyme using the isolate aspergillus niger fab211 behailu asrat1 and abebe girma2 1department of horticulture, college of agricultural sciences, arba minch university, p. Fungi isolated from sewage water of the village aspergillus niger.
A number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. The isolation of citric acid derivatives from aspergillus niger, fems microbiology letters, volume 306, issue 2, may 2010. Aspergillus commonly colonizes immunocompromised patients 3335 but can invade tissues and disseminate via the bloodstream, posing a lifethreatening. Aspergillus niger isolated from the sugarcane rhizospheric soil appeared to be an efficient phosphate solubilizers. Two isolation methods were utilized for each soil sample. Amylase, producing organisms like fungi and bacteria are generally isolated from soil and most of the work is focused on the amylase. Aspergillus secondary metabolite database, a resource to. S pg and research department of microbiology, sengamala thayaar educational trust womens college, mannargudi 614 001, tamil nadu, india.
Aspergillus niger aspergillus fumigatus penicillium chrysogenum fusarium oxysporum fig. Soil samples collected were taken to the laboratory for isolation of aspergillus niger using potato dextrose agar pda. The study is concerned with the isolation and screening of aspergillus niger strains for the biosynthesis of acid protease using solid state fermentation. Aspergillus flavus were dominantly presented in marine soil sample. From each soil sample, 10 g of soil was suspended in 90 ml of sterilized water in a 250 ml flask and was shaken for 30 min at 200 rpm. Hydrolysis of raw tuber starches by amylase of aspergillus. The number of peaks was seen to decrease with increase in incubation time and all the peaks completely disappeared at 120 h. Isolation of aspergillus niger strains from soil and their screening and optimization for enhanced citric acid production using cane molasses as carbon source. Isolation and identification of soil mycoflora in different. Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Isolation and characterization of amylase producing bacillus. Optimization of antibacterial compounds production by. Isolation of a novel strain aspergillus niger wh2 for.
To screen the xerophilic strains, the suspensions were diluted further by factors of 10. Morphological identification of aspergillus species from the soil of larkana district sindh, pakistan hina afzal 1. An agar plate medium containing bile salts and olive oil emulsion was employed for isolating and growing fungi in primary screening assay. Similarly, when the soil samples were tested for different types of fungi, penicillium, aspergillus niger, aspergillus flavus, rhizopus, fusarium oxysporoum. Balasubramanian centre of advanced study in marine biology, faculty of marine sciences, annamalai university, parangipettai608 502, cuddalore, tamil nadu, india. The soil micro fungi were enumerated by two methods, namely soil dilution 16 and soil plate. Assessment of biodegradation ability of aspergillus niger. Among the 25 isolate were identified with standard key and microbial expert. Isolation and characterization of microorganisms from.
Introduction aspergillus niger is a member of the genus aspergillus which includes a set of fungi that are generally considered asexual, although perfect forms forms that reproduce sexually have been found. Isolation and identification of antibiotic producing. The results on the aspergillus flavus was tested for. A strain of aspergillus niger was cultured from a soil sample collected from five islands provincial park, nova scotia, canada. Isolation and molecular identification of pectinase. Isolation of phosphorussolubilizing fungus from soil to. Production of acid protease by aspergillus niger using. Isolation of several species of the genus aspergillus from. The isolated keratinophilic fungi were in the following order of dominance. Isolation and characterization of various fungal strains from. Twentyone strains were selected by the ratio of the lipolytic halo radius and the colonies radius.
Eight soil samples from arable lands were collected in sterile plastic bags. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. Isolation and characterization of monocrotophos degrading. All experiments were conducted aseptically in the microbiology laboratory 10. The most frequently isolated strain was a fumigatus 129 samples, followed by a niger 75 samples.
Cadre is a repository of genomic data of aspergillus species 51. All the media ingradients and reagent chemicals were of analytical grade procured from emerck and himedia, india. Log 10 of total count cfug of aspergillus species in. Microbial degradation of lowdensity polyethylene ldpe. Aspergillus niger is a haploid filamentous fungi and is a very essential microorganism in the field of biology. The isolation of the species of the nigri section on creatine sucrose agar crea enabled to distinguish the aspergillus sp species, which was characterized by the lack of sporulation and by the. Among fifteen fungal isolates studied from these five zones aspergillus parvisclerotigenus was potent for pectinase production next to aspergillus niger in form of halozone of 0. Case report biodegradation activity of aspergillus niger. The soil fungi were isolated by the soil dilution method. Isolation of fungi seasonally from soil, air and water in villages in binary safeen. Growth of aspergillus niger was found to be optimum at 28c and ph 6. Comparative performance of aspergillus niger isolates of. Isolation and screening of aspergillus niger isolates for. A ceshproducing fungus was first isolated from soil and identified as aspergillus niger wh2 based on its morphological properties and its sequence.
This appears to be the first report of the production of methylated citric acid. A bioflocculant mbfa18 was produced by aspergillus niger a18 using potato starch wastewater psw as nutrients. Four fungal isolates from soil were screened for alpha amylase production and the isolate mjsu1101 later identified as aspergillus niger was found to have best activity among all the four isolates. Isolation and characterization of monocrotophos degrading activity of soil fungal isolate aspergillus niger mcp1 itcc7782. Present investigation deals with the isolation and screening of aspergillus niger isolates for the biosynthesis of xylanase. Various parameters of fermentation have been examined in order to improve overall enzyme yield. Optimization of citric acid production using aspergillus. Both these analyses strongly support the complete degradation of endosulfan by a. Biodegradation activity of aspergillus niger lipase isolates from a tropical country garage. The study aimed that the isolation of soil fungi from kadegaon tehsil grown in vitro during the period of august 2017. The cultivation processes and flocculating treatment for psw purification were systematically studied. Selective media were used in theisolation of aspergillus species from soil and maize kernel samples. Cellulases are the group of hydrolytic enzymes such as endoglucanase cmcase, exoglucanase.
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